Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simple image analysis for reliability control of column agglutination test in pretransfusion testing.

Transfusion medicine (Oxford, England)·2026
Same author

Dual Quantum Dot Molecular FRET Probes for Picomolar DNA Hexaplexing.

Small methods·2026
Same author

Systemic inflammatory biomarkers and their partial role in the association between diabetes and sarcopenia: A population-based study using NHANES data.

Medicine·2026
Same author

Psychosocial factors associated with depression and sleep quality in patients with tuberculosis: a multicenter cross-sectional study.

Journal of thoracic disease·2026
Same author

Multi-omics analyses reveal epigenetic regulation of anthocyanin biosynthesis in Disanthus cercidifolius subsp. longipes.

Plant physiology·2026
Same author

Cumulative Spectroscopic Detection for Taylor Dispersion Analysis of Nanoparticles.

Analytical chemistry·2026

Related Experiment Video

Updated: Dec 11, 2025

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
09:34

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Published on: February 17, 2022

3.6K

Triplexed CEA-NSE-PSA Immunoassay Using Time-Gated Terbium-to-Quantum Dot FRET.

Shashi Bhuckory1, K David Wegner2, Xue Qiu1,3

  • 1CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, 91198 Gif-sur-Yvette, France.

Molecules (Basel, Switzerland)
|August 19, 2020
PubMed
Summary
This summary is machine-generated.

This study presents a novel time-gated Förster resonance energy transfer (TG-FRET) immunoassay for simultaneously detecting three cancer biomarkers (CEA, NSE, PSA) in a single serum sample, showing clinical relevance for diagnostics.

Keywords:
CEANSEPSAbiosensingfluorescencelanthanidesmultiplexingnanoparticles

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.9K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.0K

Related Experiment Videos

Last Updated: Dec 11, 2025

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
09:34

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Published on: February 17, 2022

3.6K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.9K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.0K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Time-gated Förster resonance energy transfer (TG-FRET) using terbium (Tb) complexes and semiconductor quantum dots (QDs) offers unique photophysical properties for multiplexed biosensing.
  • Current multiplexed Tb-to-QD FRET immunoassays show promise for in vitro diagnostics but often lack the performance required for clinical applications.

Purpose of the Study:

  • To develop a homogeneous TG-FRET immunoassay capable of simultaneously quantifying three clinically relevant biomarkers: carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and prostate-specific antigen (PSA) from a single serum sample.
  • To evaluate the performance and clinical relevance of this multiplexed assay.

Main Methods:

  • Conjugation of Tb-IgG antibody donor with compact QD-F(ab')2 antibody acceptor conjugates emitting at 605, 650, and 705 nm.
  • Development of a sandwich immunoassay format where antigen binding triggers TG-FRET sensitization of QD acceptors by Tb donors.
  • Quantification of antigen concentrations based on the specific increase in FRET ratios of QD and Tb TG luminescence intensities.

Main Results:

  • Successful simultaneous quantification of CEA, NSE, and PSA from a single 50 µL serum sample.
  • Achieved limits of detection (LoDs) of 3.6 ng/mL for CEA, 3.5 ng/mL for NSE, and 0.3 ng/mL for PSA, which are within the clinically relevant range.
  • Demonstrated the feasibility of multiplexed Tb-to-QD FRET immunoassays for clinical diagnostics.

Conclusions:

  • The developed homogeneous TG-FRET immunoassay enables simultaneous detection of multiple cancer biomarkers from a single sample.
  • The assay exhibits clinically relevant sensitivity and demonstrates the potential for translation into routine clinical diagnostic platforms.
  • This multiplexed biosensing approach offers a promising advancement for in vitro diagnostics.