Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.2K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.2K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

1.4K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
1.4K
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

1.2K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
1.2K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.3K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.3K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.4K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.4K
Mass Spectrometers01:16

Mass Spectrometers

7.9K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A highly conserved basic motif in the wing domain of portal protein is necessary for oligomerization and incorporation in phage P22.

Journal of virology·2026
Same author

Negative cooperativity drives activity of capsid-directed antivirals against hepatitis B virus.

Science advances·2026
Same author

Modeling Reveals How Direct-Acting Antivirals Redirect HBV Capsid Assembly Pathways to Noninfectious Products.

bioRxiv : the preprint server for biology·2026
Same author

High resolution ES-DMA quantifies AAV capsid DNA content by electrical mobility to mass correlation.

Gene therapy·2026
Same author

Discovery and Evaluation of Cadmium-Adapted Daphnia pulex Genotypes in a Region of Historical Mining Reveals Adaptation Protects the Germline From Cadmium-Induced Mutations.

Molecular ecology·2026
Same author

Characterization of RNA using mass photometry: Method and technical perspectives.

Molecular therapy. Nucleic acids·2026

Related Experiment Video

Updated: Dec 11, 2025

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.6K

Higher Resolution Charge Detection Mass Spectrometry.

Aaron R Todd, Lauren F Barnes, Kim Young

    Analytical Chemistry
    |August 19, 2020
    PubMed
    Summary
    This summary is machine-generated.

    Charge detection mass spectrometry now achieves ten times higher mass resolving power by combining accurate charge measurements with improved m/z resolution. This advancement allows detailed analysis of complex biological assemblies like the hepatitis B virus capsid.

    More Related Videos

    Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
    11:00

    Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

    Published on: May 20, 2013

    23.2K
    Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
    12:11

    Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

    Published on: February 27, 2020

    7.2K

    Related Experiment Videos

    Last Updated: Dec 11, 2025

    Analyzing Large Protein Complexes by Structural Mass Spectrometry
    15:35

    Analyzing Large Protein Complexes by Structural Mass Spectrometry

    Published on: June 19, 2010

    24.6K
    Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
    11:00

    Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

    Published on: May 20, 2013

    23.2K
    Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
    12:11

    Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

    Published on: February 27, 2020

    7.2K

    Area of Science:

    • Analytical Chemistry
    • Biophysics
    • Mass Spectrometry

    Background:

    • Charge detection mass spectrometry (CDMS) is a single-particle technique for determining ion mass-to-charge ratio (m/z) and charge.
    • Previous CDMS mass resolving power was limited (30-40), hindering detailed analysis of complex systems.
    • Accurate mass determination is crucial for understanding the assembly of large biomolecular structures.

    Purpose of the Study:

    • To significantly improve the mass resolving power of charge detection mass spectrometry.
    • To apply the enhanced technique to analyze the assembly of the hepatitis B virus (HBV) capsid.
    • To investigate the capabilities of CDMS in resolving heterogeneous mixtures of biomolecular assemblies.

    Main Methods:

    • Implemented high-accuracy charge measurements using dynamic calibration.
    • Coupled accurate charge data with higher resolution m/z measurements within an electrostatic linear ion trap.
    • Utilized fast Fourier transformation (FFT) of time-domain signals for frequency (m/z) and magnitude (charge) analysis.

    Main Results:

    • Achieved an order of magnitude improvement in mass resolving power compared to previous CDMS.
    • Successfully resolved intermediates in hepatitis B virus (HBV) capsid assembly, from dimers to fully formed capsids.
    • Observed resonances in m/z spectra, attributed to ions with similar m/z but different masses and charges in heterogeneous samples.

    Conclusions:

    • The enhanced CDMS technique provides unprecedented mass resolution for single particles.
    • This advancement enables detailed characterization of complex, heterogeneous biomolecular assembly processes.
    • The observed resonances offer insights into the composition of heterogeneous mixtures assembled from common subunits.