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In Vivo Ear Sponge Lymphangiogenesis Assay.

Racheal G Akwii1, Md S Sajib1, Fatema T Zahra1

  • 1Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2020
PubMed
Summary

This study presents an adapted ear sponge assay for assessing in vivo lymphangiogenesis. Using immune-competent mice and Angiopoietin-2 (Ang2), it offers a reproducible method to investigate lymphatic vessel formation in inflammatory conditions.

Keywords:
Ang2Ear spongeIn vivoLymphangiogenesisLyve-1

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Area of Science:

  • Vascular Biology
  • Immunology
  • Physiology

Background:

  • Lymphangiogenesis, the formation of new lymphatic vessels, is crucial for wound healing.
  • Dysregulation of lymphangiogenesis is linked to inflammatory diseases like lymphedema and cancer metastasis.
  • Accurate in vivo models are essential for studying lymphangiogenesis and developing therapies.

Purpose of the Study:

  • To describe an adapted ear sponge assay for quantitative assessment of in vivo lymphangiogenesis.
  • To highlight a reproducible method for studying lymphatic vessel formation in an immune-competent setting.
  • To investigate the role of Angiopoietin-2 (Ang2) in lymphangiogenesis.

Main Methods:

  • Utilized an adapted ear sponge assay in immune-competent mice.
  • Employed Angiopoietin-2 (Ang2) as a key factor in the Ang/Tie signaling pathway.
  • Focused on recapitulating inflammatory conditions relevant to lymphangiogenesis.

Main Results:

  • Developed a reproducible and quantitative tool for assessing in vivo lymphangiogenesis.
  • Successfully incorporated an immune response into the experimental model.
  • Demonstrated the utility of the assay in studying lymphangiogenesis-related pathways.

Conclusions:

  • The adapted ear sponge assay provides a valuable model for studying lymphangiogenesis in an immune-competent environment.
  • This method facilitates the investigation of therapeutic targets for lymphangiogenesis-associated diseases.
  • The assay is suitable for understanding the complex regulation of lymphatic vascular remodeling.