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Updated: Dec 11, 2025
Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
A two-photon fluorescent probe for sensitive detection and imaging of γ-glutamyl transpeptidase
Ruijin Huo1, Xiuli Zheng2, Weimin Liu1
1Key Laboratory of Photochemical Conversion and Optoelectronic Materials and City U-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China. wmliu@mail.ipc.ac.cn wangpf@mail.ipc.ac.cn and School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
Abstract:
A GGT-activated two-photon fluorescent probe (4F-2CN-GSH) was developed based on a cascade reaction. 4F-2CN-GSH could selectivily detect GGT with low detection limit and distinguish ovarian cancer cells from normal cells using both one-photon and two-photon fluorescence imaging.
Insights
A novel fluorescent probe, 4F-2CN-GSH, was created to detect gamma-glutamyl transpeptidase (GGT). This probe can identify ovarian cancer cells using advanced fluorescence imaging techniques.
Area of Science:
- Biochemistry
- Chemical Biology
- Medical Imaging
Background:
- Gamma-glutamyl transpeptidase (GGT) is an enzyme linked to various physiological processes and diseases, including cancer.
- Developing selective and sensitive probes for GGT is crucial for early disease detection and diagnosis.
- Fluorescence imaging offers a powerful tool for visualizing biological processes at the cellular level.
Purpose of the Study:
- To develop a novel GGT-activated two-photon fluorescent probe for selective GGT detection.
- To evaluate the probe's performance in terms of sensitivity and specificity.
- To demonstrate the probe's utility in distinguishing ovarian cancer cells from normal cells using fluorescence imaging.
Main Methods:
- A cascade reaction was employed to synthesize the GGT-activated two-photon fluorescent probe (4F-2CN-GSH).
- The probe's fluorescence properties and selectivity for GGT were characterized.
- One-photon and two-photon fluorescence imaging were utilized to assess the probe's ability to detect GGT in ovarian cancer cells and normal cells.
Main Results:
- The developed probe (4F-2CN-GSH) demonstrated selective activation by GGT.
- The probe exhibited a low detection limit for GGT.
- Successful distinction between ovarian cancer cells and normal cells was achieved using both one-photon and two-photon fluorescence imaging.
Conclusions:
- A GGT-activated two-photon fluorescent probe (4F-2CN-GSH) was successfully developed.
- The probe offers a sensitive and selective method for GGT detection.
- This probe holds potential for fluorescence imaging-based diagnosis of ovarian cancer.
