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DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae
C N Giroux1, J R Mis, M K Pierce
1Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Molecular and Cellular Biology
|February 1, 1988
Abstract:
A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.