Related Experiment Video
Updated: Dec 11, 2025
10:12
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
Published on: December 16, 2021
3.1K
Differentiation of PTH-Expressing Cells From Human Pluripotent Stem Cells
Betty R Lawton1, Corine Martineau2, Julie Ann Sosa3
1Department of Laboratory Medicine, Yale Stem Cell Center, Yale University, New Haven, Connecticut.
Endocrinology
|August 19, 2020
Summary
Researchers developed a new method to create parathyroid-like cells from human stem cells. This breakthrough offers potential for new treatments for parathyroid disorders and drug development.
Area of Science:
- Stem cell biology
- Endocrinology
- Regenerative medicine
Background:
- Parathyroid-related disorders require effective therapeutic options.
- Current treatments for parathyroid disorders are limited.
- Pluripotent stem cells offer potential for cell-based therapies.
Purpose of the Study:
- To establish a reproducible method for differentiating pluripotent stem cells into functional parathyroid-like cells.
- To generate parathyroid hormone (PTH)-expressing cells for therapeutic applications.
- To advance the development of cell therapies for parathyroid disorders.
Main Methods:
- Directed differentiation of human embryonic stem cells and induced pluripotent stem cells.
- Comparison of various basal media formulations.
- Inclusion of CDK inhibitor PD0332991 during definitive endoderm and anterior foregut endoderm stages.
- Utilizing a two-stage pharyngeal endoderm differentiation protocol.
Main Results:
- Successfully established a reproducible directed differentiation protocol.
- Generated PTH-expressing cells from human pluripotent stem cells.
- Demonstrated the potential for developing functional parathyroid cells.
Conclusions:
- This study presents the first reproducible protocol for generating PTH-expressing cells from human pluripotent stem cells.
- The developed method is a significant step towards creating functional parathyroid cells for therapeutic use.
- The findings have broad applicability for medicinal and scientific investigation in parathyroid disorders.
Related Concept Videos
01:22iPS Cell Differentiation
3.0K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.0K
01:13Induced Pluripotent Stem Cells
26.7K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
26.7K