A DCM-based NIR sensor for selective and sensitive detection of Zn²⁺ in living cells

Zinc ion is the 2nd abundant transition metal element in human's body. It is responsible for many physiological and biological functioning in the body, such as growth of people, immunity, endocrine, etc. The deficiency of zinc could result in an increasing risk for growth retardation, neurological disorder and infectious disease. Thus, developing a nondestructive method for detecting Zn²⁺ in living systems is important. Here we reported a 2-(2-methyl-4H-ylidene)- malononitrile (DCM)-based NIR probe DF-Zn for selective and sensitive detection of Zn²⁺. The probe DF-Zn is cell-permeable and stable at broad pH range. DF-Zn showed a fast response to Zn²⁺, big stock's shift, and "nude-eye" recognition for Zn²⁺. Moreover, the selective binding of probe DF-Zn to Zn²⁺ was reversible. With the addition of EDTA in buffer solution, reversible response of probe to Zn²⁺ could be observed in MCF-7 cells imaging.