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Updated: Dec 11, 2025

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
Published on: April 3, 2014
Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells
Mikkel R Holst1, Simone S E Nielsen1, Morten S Nielsen2
1Institute of Biomedicine, Aarhus University, Aarhus C, Denmark.
Abstract:
Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ.
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