Related Experiment Video
Updated: Dec 11, 2025
11:27
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Published on: August 28, 2018
22.8K
Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9
Francisco Carmona-Aldana1, Hober N Nuñez-Martinez1, Carlos A Peralta-Alvarez1
1Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2020
Summary
This study details a CRISPR-Cas9 genome editing protocol for zebrafish embryos. This method enables precise DNA sequence modifications in vivo for research applications.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- CRISPR-Cas9 is a powerful genome editing tool.
- In vivo applications require efficient and precise editing methods.
Purpose of the Study:
- To describe a protocol for CRISPR-Cas9 genome editing.
- To enable site-directed sequence eliminations in zebrafish embryos.
Main Methods:
- CRISPR-Cas9 system implementation.
- Application in zebrafish (Danio rerio) one-cell embryos.
Main Results:
- Successful genome editing in zebrafish embryos.
- Generation of site-directed sequence eliminations.
Conclusions:
- The described protocol facilitates efficient in vivo genome editing in zebrafish.
- This method is valuable for genetic research and functional studies in zebrafish models.
