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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Related Experiment Video

Updated: Dec 11, 2025

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
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Epitranscriptomics in the Heart: a Focus on m6A.

Jacob Z Longenecker1, Christopher J Gilbert1, Volha A Golubeva1

  • 1Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, 473 W 12th Ave, Columbus, OH, 43210, USA.

Current Heart Failure Reports
|August 20, 2020
PubMed
Summary
This summary is machine-generated.

N6-methyladenosine (m6A) is a crucial RNA modification impacting gene expression and cellular responses. This review highlights m6A

Keywords:
EpigeneticsHeart failureRNA methylationm6A

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cardiovascular Research

Background:

  • Post-transcriptional modifications regulate gene expression in response to environmental stimuli.
  • N6-methyladenosine (m6A) is the most abundant internal mRNA modification, influencing RNA splicing, localization, translation, and decay.
  • m6A has been linked to various diseases, with emerging evidence in cardiac pathologies.

Purpose of the Study:

  • To review recent evidence on the involvement of m6A in cardiac pathologies and processes.
  • To explore the role of m6A in the heart's response to various stress conditions.

Main Methods:

  • Studies utilized gain and loss of function models for m6A-modifying enzymes.
  • Genome-wide analyses identified differentially methylated mRNAs during cardiac stress.

Main Results:

  • m6A plays a multifaceted role in the heart's response to myocardial infarction, pressure overload, and ischemia/reperfusion injuries.
  • m6A regulates the translation of transcripts involved in calcium handling, cell growth, autophagy, and adrenergic signaling in cardiomyocytes.
  • Differential methylation of mRNAs during cardiac stress underscores m6A's importance.

Conclusions:

  • Regulation of gene expression by m6A is critical for cardiomyocyte homeostasis and stress responses.
  • m6A modification plays a key role in cardiac pathophysiology.