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Quantitative electron energy loss spectroscopy in biology
1Biomedical Engineering and Instrumentation Branch, National Institutes of Health, Bethesda, Maryland 20892.
Ultramicroscopy
|January 1, 1988
Summary
Electron energy loss spectroscopy (EELS) offers powerful elemental analysis for biological samples. This technique enables detailed composition and distribution mapping of light elements in cells, advancing biological research.
Area of Science:
- Materials Science
- Biophysics
- Analytical Chemistry
Background:
- Electron energy loss spectroscopy (EELS) is a microscopy technique.
- Recent advancements in instrumentation and detection have improved EELS capabilities.
- EELS has potential applications in biological research.
Purpose of the Study:
- To assess the applicability of EELS in biological studies.
- To highlight recent instrumentation developments for EELS.
- To demonstrate the quantitative capabilities of EELS for elemental analysis in biological samples.
Main Methods:
- Utilized EELS for elemental analysis and mapping.
- Employed parallel detection and spectrometer design improvements.
- Analyzed DNA, insulin, histidine, and cryosectioned chromaffin cells.
- Performed quantitative elemental mapping in STEM mode.
Main Results:
- Demonstrated accurate N:P ratio in DNA using EELS.
- Tabulated biologically relevant elements and their applications.
- Illustrated detection limits and radiation damage effects.
- Determined water content and N/P ratios in chromaffin cells.
- Obtained quantitative elemental maps of light elements in cells.
Conclusions:
- EELS is a viable technique for elemental analysis in biology.
- Advancements in EELS instrumentation enhance its quantitative capabilities.
- EELS provides insights into cellular composition and element distribution.