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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

939
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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Highly selective and sensitive chemosensor for Al(III) based on isoquinoline Schiff base.

Yu-Ying Huang1, Feng-Xue Wang2, Si-Yu Mu3

  • 1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, PR China; School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|August 20, 2020
PubMed
Summary

A new sensor, N'-(2-hydroxybenzylidene)isoquinoline-3-carbohydrazide (HL), was synthesized for detecting aluminum ions (Al3+). This fluorescent sensor shows a significant increase in signal and can monitor intracellular Al3+ levels.

Keywords:
Al(III)Cell imagingDFTIsoquinolineSchiff baseTurn-on fluorescence sensor

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biochemistry

Background:

  • Aluminum ions (Al3+) play crucial roles in biological processes.
  • Developing selective and sensitive sensors for Al3+ is essential for research and diagnostics.
  • Existing methods for Al3+ detection may lack sensitivity or selectivity.

Purpose of the Study:

  • To synthesize and characterize a novel colorimetric and fluorescent sensor for Al3+ detection.
  • To evaluate the sensor's performance, including sensitivity, selectivity, and reversibility.
  • To explore the sensor's application in biological imaging for intracellular Al3+ monitoring.

Main Methods:

  • Synthesis of N'-(2-hydroxybenzylidene)isoquinoline-3-carbohydrazide (HL).
  • Spectroscopic analysis (fluorescence, colorimetric) for Al3+ detection.
  • Metal ion interference studies and reversibility tests using EDTA.
  • Electrospray ionization mass spectrometry (ESI-MS) and Job's plot analysis for complex stoichiometry.
  • Density functional theory (DFT) calculations.
  • Cell imaging experiments.

Main Results:

  • HL was successfully synthesized as a turn-on sensor for Al3+.
  • A 273-fold increase in fluorescence intensity at 458 nm was observed upon Al3+ binding.
  • The limit of detection for Al3+ was determined to be 1.11 × 10^-9 M.
  • The sensor exhibited high selectivity for Al3+ over other metal ions and demonstrated reversible sensing capabilities.
  • A 2:1 coordination complex between HL and Al3+ was identified.
  • HL was successfully applied for monitoring intracellular Al3+ levels in cell imaging.

Conclusions:

  • The synthesized HL acts as a highly sensitive and selective colorimetric and fluorescent sensor for Al3+.
  • The sensor's ability to detect Al3+ visually and its reversible nature make it a promising tool.
  • HL demonstrates potential for real-time monitoring of intracellular Al3+ concentrations in biological systems.