Related Experiment Video
Updated: Dec 11, 2025
Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
Published on: August 16, 2017
The effect of fibrin formulation on cell migration in an in vitro tendon repair model
Kosuke Uehara1, Chunfeng Zhao1, Anne Gingery1
1Orthopaedic Biomechanics and Tendon and Soft Tissue Biology Laboratories, Division of Orthopedic Research, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA.
Background:
The purpose of this study was to determine the effect of fibrinogen concentration on cell viability and migration in a tissue culture tendon healing model.
Methods:
Forty-eight canine flexor digitorum profundus tendons were randomly divided into three groups. In each group the tendons were lacerated and repaired augmented with a canine bone marrow stromal cell seeded fibrin interposition patch using either 5 mg/ml fibrinogen and 25 U/ml thrombin (physiological as a control), 40 mg/ml fibrinogen and 250 U/ml thrombin (low adhesive), or 80 mg/ml fibrinogen and 250 U/ml thrombin (high adhesive). The sutured tendons were cultured for two or four weeks.
Results:
Failure load was not significantly different among the groups. Cell-labeling staining showed that the stromal cells migrated across the gap in the control and low adhesive groups, but there was no cell migration in the high adhesive group at two weeks.
Conclusion:
A high fibrinogen concentration in a fibrin patch or glue may impede early cell migration.
Level Of Evidence:
Not applicable because this study was a laboratory study.
