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Updated: Dec 11, 2025

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
Published on: May 15, 2018
Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant
So-Ichiro Nishiyama1, Yoshiaki Hasegawa2, Keiji Nagano3
1Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan. snishiyama@nupals.ac.jp.
Abstract:
Porphyromonas gingivalis, an etiological agent of chronic periodontitis, is an asaccharolytic anaerobic gram-negative coccobacillus. Genetic approaches greatly facilitate research on organisms at the molecular level. Although with some challenges, the use of genetic techniques (such as constructing knockout mutants) in P. gingivalis are feasible. In this chapter, we describe detailed methods for site-directed and random mutagenesis through the construction of fimbriae-related gene mutants of P. gingivalis.
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