Related Experiment Video
Updated: Dec 11, 2025

11:35
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
8.5K
Transcriptomic dataset of wild type and phoP mutant Pectobacterium versatile.
Natalia Gogoleva1, Uljana Kravchenko2, Yevgeny Nikolaichik2
1Kazan Institute of Biochemistry and Biophysics, Federal Research Center "Kazan Scientific Center of RAS", Kazan, Russia.
Data in Brief
|August 21, 2020
Summary
This study details RNA-Seq data for Pectobacterium versatile, revealing genes controlled by the PhoP system. This helps understand how this plant pathogen adapts to its environment.
Area of Science:
- Microbiology
- Plant Pathology
- Genomics
Background:
- Pectobacterium versatile is a plant pathogenic bacterium.
- The PhoP/PhoQ system is a key environmental sensor in bacteria.
- Understanding P. versatile adaptation requires knowledge of its regulatory systems.
Purpose of the Study:
- To describe RNA-Seq transcriptome data for wild type and phoP mutant P. versatile strains.
- To identify genes transcriptionally regulated by the PhoP system in P. versatile.
- To elucidate the role of PhoP in the physiology and adaptation of this plant pathogen.
Main Methods:
- RNA-Sequencing (RNA-Seq) was performed on wild type and phoP mutant strains of P. versatile.
- Transcriptome data analysis was conducted to identify differentially expressed genes.
- Comparative genomics approaches may be employed to analyze the PhoP regulon.
Main Results:
- The dataset provides comprehensive transcriptome profiles for P. versatile under specific conditions.
- Identification of genes directly or indirectly controlled by PhoP is enabled by this data.
- The data lays the groundwork for understanding PhoP-mediated regulation in P. versatile.
Conclusions:
- This RNA-Seq dataset is a valuable resource for studying P. versatile pathogenesis.
- The findings will advance our understanding of the PhoP/PhoQ system's role in plant pathogens.
- Further research can utilize this data to explore P. versatile adaptation mechanisms.
Related Concept Videos
Transcription Attenuation in Prokaryotes
17.8K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
17.8K
Mutations in Microorganisms
392
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
392
Transcription in Prokaryotes
1.5K
Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow...
1.5K

