VEGF/CDK2 are involved in diabetic organ regeneration
Rashmi K Ambasta1, Krishna Adeshara2, Shivangi Yadav3
1Molecular Neuroscience and Functional Genomics Lab, Delhi Technological University, Delhi, India; School of Biosciences and Technology (SBST), Vellore Institute of Technology, Vellore, India.
Aim/Hypothesis:
Diabetes is a hyperglycaemic disease treated by a set of allopathic drugs and natural biomolecules along with many variety of stem cell. We aim to investigate the role of these drugs in targeting common protein molecule in diabetes and its associated disease. We also aim to investigate the organ degeneration mechanistic pathway in diabetes.
Method:
We have generated diabetes using streptozotocin injection and treated them using bone marrow transplantation and curcumin administration. The organs were studied histopathologically and by immunofluorescence analysis while drugs were studied Pharmacogenomically.
Result:
Mice injected with streptozotocin have higher glucose and lower insulin, islet number/diameter, bone marrow cell number compared to control and bone marrow transplanted and curcumin administered mice. Histopathology staining demonstrates damaged morphology of pancreas, kidney, brain and cardiac muscle. Further, upon comparison of all allopathic and ayurvedic drugs used for diabetes several protein targets have been identified by reverse pharmacophore analysis using PharmMapper. VEGF, CDK2, insulin receptor, HSp90, eNOS, Fructose1,6 bisphosphatase, neprilysin, AchE, MAPK are several common protein targets of anti-diabetic drugs.
Conclusion:
This article demonstrates that VEGF and CDK2 are critical marker in organ damage in diabetes as well as organ regeneration.
Insights
This study reveals that vascular endothelial growth factor (VEGF) and cyclin-dependent kinase 2 (CDK2) are key markers in diabetes-related organ damage and regeneration. These findings offer insights into potential therapeutic targets for managing diabetes complications.
Area of Science:
- Biochemistry
- Pharmacology
- Diabetology
Background:
- Diabetes mellitus is a complex hyperglycemic condition managed through allopathic drugs, natural compounds, and stem cell therapies.
- Understanding the molecular mechanisms of organ degeneration in diabetes is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the common protein targets of anti-diabetic drugs.
- To explore the mechanistic pathways of organ degeneration in diabetes.
- To identify key molecular markers for organ damage and regeneration in diabetes.
Main Methods:
- Diabetes was induced in mice using streptozotocin and treated with bone marrow transplantation and curcumin.
- Organs were analyzed using histopathology and immunofluorescence.
- Drug targets were identified using reverse pharmacophore analysis (PharmMapper).
Main Results:
- Streptozotocin-induced diabetes resulted in hyperglycemia, reduced insulin, and damaged pancreatic, kidney, brain, and cardiac tissues.
- Common protein targets for anti-diabetic drugs include VEGF, CDK2, insulin receptor, HSp90, eNOS, and others.
- VEGF and CDK2 were identified as critical markers in diabetes-induced organ damage and regeneration.
Conclusions:
- VEGF and CDK2 play critical roles in both the pathogenesis and potential regeneration of organs affected by diabetes.
- Identifying common drug targets provides a basis for developing more targeted and effective diabetes therapies.
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