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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
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Protein-Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles
1College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon 21936, Korea.
International Journal of Nanomedicine
|August 22, 2020
Summary
Nanoparticles (NPs) interact with proteins in vivo, forming a "protein corona." Understanding protein conformational changes on NPs is crucial for safe and effective biomedical applications.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Biochemistry
Background:
- Nanoparticles (NPs) show promise for disease diagnosis and targeted drug delivery.
- In vivo NP applications face challenges like toxicity, unpredictable distribution, and poor targeting.
- The protein corona, formed by proteins adsorbing onto NPs, significantly impacts NP behavior.
Purpose of the Study:
- To elucidate the characteristics of the protein corona.
- To investigate the conformational fluctuations of corona proteins induced by NPs.
- To highlight the importance of understanding protein structure changes for NP applications.
Main Methods:
- Review of existing literature on protein corona formation and characteristics.
- Focus on experimental observations of protein conformational changes upon NP adsorption.
- Analysis of the kinetics and dynamics of protein-NP interactions.
Main Results:
- Protein adsorption onto NPs forms a dynamic "protein corona."
- Conformational changes and unfolding of adsorbed proteins are common.
- These structural alterations significantly influence NP biological behavior and functionality.
Conclusions:
- Understanding protein corona formation and its impact on protein structure is vital.
- Investigating NP-induced protein conformational changes is key to advancing NP-based therapies.
- Addressing protein corona effects will improve the safety and efficacy of nanomedicines.
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