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Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
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Stromal Nerve Imaging and Tracking Using Micro-Optical Coherence Tomography.

Carolin Elhardt1,2, Christian M Wertheimer1,2, Andreas Wartak1,3

  • 1Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.

Translational Vision Science & Technology
|August 22, 2020
PubMed
Summary

Micro-optical coherence tomography (µOCT) successfully imaged and tracked corneal nerves in rabbit eyes. This technique shows promise for a noncontact screening tool for early peripheral neuropathy.

Keywords:
corneal nervesdiabetesimagingmicro-OCToptical coherence tomography

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Area of Science:

  • Ophthalmology
  • Biomedical Imaging
  • Neuroscience

Background:

  • Peripheral neuropathy can affect corneal nerves, serving as an early indicator of systemic disease.
  • Accurate imaging of corneal nerve structures is crucial for early diagnosis and monitoring.

Purpose of the Study:

  • To develop a screening tool for early peripheral neuropathy by imaging and mapping nerve fiber distribution in rabbit corneas.
  • To utilize micro-optical coherence tomography (µOCT) for visualizing nerve fibers throughout the entire stromal thickness.

Main Methods:

  • Excised rabbit corneas were imaged using a custom µOCT prototype and a confocal microscope.
  • µOCT images (1 µm axial, 4 µm transverse resolution) were processed with semiautomatic algorithms to segment nerve structures.
  • Validation was performed by comparing µOCT data with βIII-tubulin antibody immunostaining and confocal microscopy.

Main Results:

  • Semiautomatic algorithms enabled tracing of nerves with varying thicknesses across the full corneal volume.
  • A strong correlation was observed between hyper-reflective structures in µOCT data and nerval structures identified via immunohistochemistry.
  • µOCT successfully mapped nerve fiber distribution throughout the entire corneal stroma.

Conclusions:

  • This study demonstrates the capability of µOCT for ex vivo nerval imaging, tracking, and spatial correlation with established corneal nerve imaging standards.
  • µOCT provides a noncontact method for assessing corneal nerval structures, potentially aiding in the early detection of peripheral neuropathy.