Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PDE5a Inhibition Restricts Cancer Metastasis by Disrupting NPC1-Mediated Cholesterol Trafficking Through a Non-canonical cGMP-Dependent Pathway.

Cancer research·2026
Same author

Piezoelectricity from Dopant-Induced Structural Distortions in Molecular Crystals Revealed by Raman Spectroscopy.

Journal of the American Chemical Society·2026
Same author

Disruption of the brain-spleen axis impairs monocyte-microglia communication and accelerates disease progression in a mouse model of amyloidosis.

Nature communications·2026
Same author

Biallelic rescue of CTG18.1 in two Fuchs endothelial corneal dystrophy-derived iPSC lines (SCTCi047-A-2, SCTCi046-A-2) following a two-step gene editing strategy.

Stem cell research·2026
Same author

Scleral remodeling in myopia: mechanisms and therapeutic approaches.

Progress in retinal and eye research·2026
Same author

Parsing the functions of immediate-early proteins in the lytic-latent balance of HCMV infection.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Dec 11, 2025

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis
07:29

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Published on: November 12, 2015

20.3K

Decreased Riboflavin Impregnation Time Does Not Increase the Risk for Endothelial Phototoxicity During Corneal

Arie L Marcovich1,2, Jurriaan Brekelmans1,3, Alexander Brandis1,4

  • 1Plant and Environmental Sciences Department, Weizmann Institute of Science, Rehovot, Israel.

Translational Vision Science & Technology
|August 22, 2020
PubMed
Summary

Shortening riboflavin (RF) impregnation time during corneal crosslinking (CXL) is safe. Reduced impregnation time decreases patient burden without increasing endothelial cell damage risk from UVA light.

Keywords:
corneal collagen cross-linkingendothelial safetykeratoconusriboflavin

More Related Videos

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
05:56

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Published on: April 3, 2016

9.2K
Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

2.1K

Related Experiment Videos

Last Updated: Dec 11, 2025

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis
07:29

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Published on: November 12, 2015

20.3K
Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
05:56

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Published on: April 3, 2016

9.2K
Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

2.1K

Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Corneal Science

Background:

  • Corneal crosslinking (CXL) is a standard treatment for progressive keratoconus.
  • Riboflavin (RF) impregnation is a crucial step in CXL, requiring significant patient time.
  • Optimizing RF impregnation duration is essential for patient comfort and operational efficiency.

Purpose of the Study:

  • To evaluate riboflavin (RF) concentration and distribution in the corneal stroma.
  • To assess the risk of endothelial photodamage during corneal crosslinking (CXL) after 10- and 30-minute RF impregnation.
  • To determine the impact of shortened RF impregnation on corneal endothelial cells.

Main Methods:

  • De-epithelialized rabbit corneas underwent 10- and 30-minute RF impregnation with varying formulations.
  • Human corneal endothelial cells (HCECs) were exposed to different RF concentrations and UVA dosages.
  • Fluorescence imaging, absorption spectroscopy, and cell viability staining were employed.

Main Results:

  • Anterior chamber fluid RF concentration increased with impregnation time (10 min: 1.6×10-4%, 30 min: 5.4×10-4%).
  • Endothelial RF concentration decreased from 0.019% to 0.0056% with shortened impregnation, while UVA irradiance increased 1.3-fold.
  • In vitro HCEC viability was not statistically different between 10-minute (51.0% ± 3.9%) and 30-minute (41.3% ± 5.0%) impregnation groups.

Conclusions:

  • Shortened RF impregnation (10 minutes) does not increase endothelial damage risk in CXL.
  • Reduced impregnation time is accompanied by decreased posterior stromal RF concentration, mitigating potential damage.
  • In vitro results suggest 10-minute impregnation may be favorable, supporting reduced patient burden and OR time.