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Spermatogenesis is normal in Tex33 knockout mice.

Zhendong Zhu1, Xin Zhang1, Wentao Zeng2

  • 1Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.

Peerj
|August 22, 2020
PubMed
Summary

Testis expressed gene 33 (Tex33) is not essential for male fertility or spermatogenesis in mice. Tex33 knockout mice show normal sperm parameters and reproductive success, indicating its redundant role in the process.

Keywords:
Male infertilityMouseNIRH and SYT domainSpermatogenesisTex33

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Area of Science:

  • Reproductive biology
  • Genetics
  • Molecular biology

Background:

  • Testis expressed gene 33 (Tex33) is a recently identified, evolutionarily conserved, testis-specific gene.
  • Tex33 expression is localized to the cytoplasm of round spermatids in mice.
  • The in vivo function of Tex33 in spermatogenesis remains largely unknown.

Purpose of the Study:

  • To investigate the in vivo function of Tex33 in male mouse fertility and spermatogenesis.
  • To determine if Tex33 plays a critical role in the process of sperm formation.

Main Methods:

  • Generation of Tex33 knockout (Tex33-/-) mice using CRISPR/Cas9 technology with a 62bp in-frame deletion in Exon2.
  • Phenotypic analysis of Tex33-/- adult male mice, including fertility, litter size, testis/body weight ratios, tissue morphology, sperm count, morphology, and motility.
  • Assessment of germ cell apoptosis using TUNEL assay and evaluation of the first wave of spermiogenesis in 5-week-old mice, examining key spermatogenesis markers (PLZF, γ-H2AX, TNP1).

Main Results:

  • Tex33-/- adult male mice were fertile with no significant changes in litter size compared to wild-type (Tex33+/+) littermates.
  • No significant differences were observed in testis/body weight ratios, testicular/epididymal morphology, sperm count, sperm morphology, or sperm motility between Tex33-/- and Tex33+/+ mice.
  • Germ cell apoptosis ratios and the first wave of spermiogenesis, including the formation of elongating spermatids and the expression of PLZF, γ-H2AX, and TNP1, were not disrupted in Tex33-/- mice.

Conclusions:

  • Tex33 is a redundant gene with no essential role in male fertility or spermatogenesis in mice.
  • This finding can help researchers avoid redundant studies on the Tex33 gene.
  • Further research may explore potential subtle roles or alternative functions of Tex33 in other biological contexts.