Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis
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Updated: Dec 11, 2025

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Published on: August 16, 2016
Romualdas Vaisvila1, Lise Hunault1,2, Lana Saleh3
1New England Biolabs, Inc., Ipswich, MA, USA.
Abstract:
The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high throughput capillary gel electrophoresis assay for monitoring the in vitro oxidation of 5mC by TET. The method is rapid and quantitative, and can serve as a powerful tool in mechanistic studies of TET.
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