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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Published on: August 16, 2016
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Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brain
Marcus D Wallis1, Rebecca C Trueman2
1School of Life Sciences, University of Nottingham, Nottingham, UK.
Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2020
Abstract:
The modified cytosine base 5-hydroxymethylcytosine (5hmC) is abundantly present in the central nervous system (CNS), and visualization of global 5hmC levels is possible through use of immunohistochemistry. In this chapter we describe an adaptable method of brain tissue collection and immunohistochemical staining that allows for detection of 5hmC in mouse or rat brain, meaning that the method can be applied to many rodent models of CNS diseases and disorders.
