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Methylated DNA Immunoprecipitation
Published on: January 2, 2009
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Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing
Millissia Ben Maamar1, Ingrid Sadler-Riggleman1, Daniel Beck1
1Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2020
Abstract:
Methylated DNA immunoprecipitation is a large scale purification technique. It enables the isolation of methylated DNA fragments for subsequent locus-specific or genome-wide analysis. Here we describe an immunoprecipitation protocol using a monoclonal mouse anti 5-methyl-cytidine antibody followed by next-generation sequencing (MeDIP-Seq).
