Related Experiment Video
Updated: Dec 11, 2025
08:38
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
Published on: March 3, 2015
13.7K
A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions
Woj M Wojtowicz1, Jost Vielmetter2, Ricardo A Fernandes1
1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Cell
|August 22, 2020
Summary
Researchers mapped over 380 novel cell-surface protein-protein interactions (PPIs), revealing a complex communication network. This discovery enhances understanding of immune and nervous system functions and identifies potential therapeutic targets.
Area of Science:
- Cell Biology
- Immunology
- Neuroscience
- Proteomics
Background:
- Cell-surface protein-protein interactions (PPIs) are crucial for cell-cell communication, recognition, and responses.
- Understanding these interactions is vital for deciphering biological processes and disease mechanisms.
Purpose of the Study:
- To systematically screen for novel human cell-surface and secreted protein-protein interactions (PPIs).
- To identify new interaction partners for both known and orphan receptors.
- To build a comprehensive interactome map of cell-surface proteins.
Main Methods:
- A high-throughput, automated ELISA-based screening platform was utilized.
- An interactome screen of 564 human cell-surface and secreted proteins, predominantly immunoglobulin superfamily (IgSF) proteins, was performed.
- Phylogenetic homology analysis, surface plasmon resonance, and cell binding assays were employed for validation.
Main Results:
- The screen identified approximately 380 previously unreported PPIs among 318,096 tested combinations.
- New interactions were found for receptors with known ligands and for "orphan" receptors.
- Identified PPIs involve proteins in diverse functions, including immune and nervous system development, metabolism, and reproduction.
Conclusions:
- A vast and intricate network of cell-surface PPIs has been uncovered.
- These novel interactions provide a valuable resource for investigating biological functions.
- The identified PPIs represent potential targets for therapeutic drug development.
Related Concept Videos
02:04Protein-protein Interfaces
14.3K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.3K
02:04Protein-Protein Interfaces
4.3K
4.3K
02:26Protein Networks
4.4K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.4K
02:26Protein Networks
2.7K
2.7K
01:30Assembly of Signaling Complexes
6.3K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.3K
01:57Protein Complexes with Interchangeable Parts
2.8K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.8K