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Fe-S cluster biogenesis by the bacterial Suf pathway.

Matthew Blahut1, Enis Sanchez1, Claire E Fisher1

  • 1Department of Chemistry and Biochemistry, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, USA.

Biochimica Et Biophysica Acta. Molecular Cell Research
|August 22, 2020
PubMed
Summary

Iron-sulfur (FeS) cluster biogenesis is vital for cell function. The ancient sulfur formation (Suf) pathway, crucial in bacteria, is a target for new antibacterial drugs.

Keywords:
A-type carrier proteinIronIron-sulfur clusterMetallocofactorSuf

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Area of Science:

  • Cellular Biology
  • Biochemistry
  • Microbiology

Background:

  • Iron-sulfur (FeS) clusters are essential for numerous cellular functions.
  • FeS cluster biogenesis involves complex multi-protein machinery for handling reactive intermediates.
  • The ancient sulfur formation (Suf) pathway is conserved in bacteria and archaea.

Purpose of the Study:

  • To summarize recent advancements in understanding the bacterial Suf pathway for FeS cluster biogenesis.
  • To highlight the Suf pathway's role in pathogenic bacteria as a potential drug target.

Main Methods:

  • Characterization of the SufS-SufE/SufU complex in sulfur mobilization and protection.
  • Analysis of FeS cluster assembly on the SufBC2D scaffold.
  • Investigation of nascent FeS cluster trafficking to A-type carrier proteins.

Main Results:

  • Progress in understanding sulfur mobilization and protection by SufS-SufE/SufU.
  • Characterization of FeS cluster assembly on the SufBC2D scaffold.
  • Insights into the trafficking of FeS clusters to carrier proteins.

Conclusions:

  • The Suf pathway is critical for FeS cluster biogenesis in bacteria, particularly in pathogens.
  • Understanding the Suf pathway provides targets for novel antibacterial therapies.