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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

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Surface-modified nanoerythrosomes for potential optical imaging diagnostics.

Marco Fornasier¹, Andrea Porcheddu², Anna Casu³

¹Department of Chemical and Geological Sciences, University of Cagliari, s.s. 554 bivio Sestu, I-09042 Monserrato, Cagliari, Italy; CSGI, Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase, Via della Lastruccia 3, I-50019 Sesto Fiorentino, Florence, Italy.

Journal of Colloid and Interface Science

|August 22, 2020

Summary

This summary is machine-generated.

Nanoerythrosomes (NERs), derived from red blood cells, show promise as diagnostic tools. Functionalized NERs with fluorophores demonstrate stability and efficiency for in vitro/in vivo imaging applications.

Keywords:

Cu-free click chemistry Fluorescence Ghosts Red blood cells Vesicles

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Area of Science:

Biotechnology
Nanomedicine
Biocompatible materials

Background:

Nanoerythrosomes (NERs) are vesicle-like nanoparticles derived from red blood cells.
NERs offer high stability and excellent biocompatibility, making them suitable for therapeutic and imaging applications.

Purpose of the Study:

To demonstrate the proof-of-concept for using NERs as diagnostic tools for in vitro/in vivo imaging.
To functionalize NER surfaces with different fluorophores for imaging purposes.

Main Methods:

NERs derived from bovine blood were functionalized with two fluorophores: 7-amino-4-methylcumarin and dibenzocyclooctinecyanine5.5.
Fluorophores were attached to the NER surface using glutaraldehyde cross-linking and click chemistry (strain-promoted azide-alkyne cycloaddition).
Physicochemical characterization and loading efficiency were assessed using UV-Vis spectroscopy and fluorescence microscopy.

Main Results:

Functionalized NERs exhibited high stability under physiological conditions.
The study successfully demonstrated the attachment of fluorophores to the NER surface.
Loading efficiency of fluorophores was quantitatively evaluated.

Conclusions:

Functionalized NERs are stable and can be efficiently loaded with imaging probes.
This study provides a foundation for utilizing NERs as diagnostic imaging agents.