Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tailoring Molecular Architectures for Intramolecular Charge Separation of the Multiexciton State Generated by Singlet Fission.

Journal of the American Chemical Society·2026

Same author

Redox- and Photo-Responsive Fe<sup>3+/2+</sup>-Cross-Linked Carboxymethyl Cellulose Methacrylate Dissipative Gels: Synthesis and Applications.

ACS applied materials & interfaces·2026

Same author

Early versus delayed laparoscopic cholecystectomy following endoscopic retrograde cholangiopancreatography for concomitant gallstones and common bile duct stones: a retrospective study.

BMC surgery·2026

Same author

Tuning Quintet Multiexciton Sublevel Selection via Bridge Resonance in Singlet Fission.

The journal of physical chemistry letters·2026

Same author

Solid Pro-Nano Lipid Oral Formulations for Cannabidiol (CBD).

Pharmaceutics·2026

Same author

Controlling triplet-pair formation in acene-bridged trimers through locally excited-charge-transfer state mixing.

Chemical science·2026

Search research articles

Related Experiment Video

Updated: Dec 11, 2025

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Cyclopropenium Nanoparticles and Gene Transfection in Cells.

Noam Y Steinman¹, Luis M Campos², Yakai Feng³

¹Institute of Drug Research, School of Pharmacy-Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel.

|August 23, 2020

Summary

New cyclopropenium nanoparticles offer enhanced gene transfection with minimal toxicity, presenting a promising alternative to traditional non-viral vectors for advanced gene therapy applications.

Keywords:

cationic nanoparticles cyclopropenium gene transfection poly(ethylene imine)

More Related Videos

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Related Experiment Videos

Last Updated: Dec 11, 2025

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Area of Science:

Biomaterials Science
Gene Therapy
Nanotechnology

Background:

Non-viral vectors are crucial for delivering genetic material in gene therapy.
Current methods often face challenges with efficiency and toxicity.
Quaternary ammonium compounds are common but have limitations.

Purpose of the Study:

To introduce cyclopropenium-based nanoparticles as novel non-viral gene transfection agents.
To evaluate their efficiency and cytotoxicity compared to existing methods.
To explore their potential in gene therapy applications.

Main Methods:

Synthesized cyclopropenium-based cationic nanoparticles by crosslinking poly(ethylene imine) (PEI) with tetrachlorocyclopropene.
Complexed nanoparticles with plasmid DNA, forming ~50 nm structures.
Assessed cellular uptake and gene expression in F929 mouse fibroblast cells in vitro.

Main Results:

Cyclopropenium nanoparticles demonstrated enhanced gene transfection compared to PEI.
These nanoparticles exhibited minimal cytotoxicity.
Efficient cellular uptake and gene expression were observed in vitro.

Conclusions:

Cyclopropenium-based nanoparticles are effective non-viral gene delivery vectors.
They offer a promising alternative with improved transfection efficiency and reduced toxicity.
These findings highlight their potential for future gene therapy development.