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The Circulation of Type F Clostridium perfringens among Humans, Sewage, and Ruditapes philippinarum (Asari Clams)

Keita Yanagimoto1,2, Kosei Uematsu1, Takaya Yamagami1

  • 1Department of Microbiology, Yamanashi Institute of Public Health and Environment, 1-7-31 Fujimi, Kofu, Yamanashi 400-0027, Japan.

Pathogens (Basel, Switzerland)
|August 23, 2020
PubMed

Insights

Clostridium perfringens (C. perfringens) circulates between humans, sewage, and asari clams. Asari clams are identified as a key source of C. perfringens, contributing to foodborne illness and environmental contamination.

Area of Science:

  • Environmental microbiology
  • Food safety
  • Bacteriology

Background:

  • Clostridium perfringens (C. perfringens) causes gastroenteritis via enterotoxin (CPE).
  • Shellfish can accumulate C. perfringens from aquatic environments.
  • Sewage pollution poses a risk for pathogen transmission.

Purpose of the Study:

  • To investigate the circulation of type F C. perfringens.
  • To assess C. perfringens in humans, sewage, and asari clams (Ruditapes philippinarum).
  • To understand the role of asari clams in C. perfringens transmission.

Main Methods:

  • Serotyping and genotyping (cpe, toxin) of C. perfringens isolates.
  • Plasmid IS1151 sequence analysis.
  • Multilocus sequence typing (MLST) for isolate comparison.

Main Results:

  • C. perfringens with plasmid IS1151 downstream of cpe was predominant across all sample types.
  • Isolates from humans, sewage, and asari clams showed genetic links via MLST.
  • Asari clams were identified as a significant source of C. perfringens.

Conclusions:

  • Asari clams serve as a crucial infection source for C. perfringens carriers and foodborne diseases.
  • Human infections linked to asari clams can lead to further environmental contamination.
  • Monitoring C. perfringens in sewage aids in assessing community-level pathogen trends.
Keywords:
Ruditapes philippinarumasari clamcpe-positive C. perfringensmultilocus sequence typingwastewater treatment plant

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