Recent developments in the use of peroxygenases - Exploring their high potential in selective oxyfunctionalisations.

Area of Science:

• Biocatalysis
• Organic Synthesis
• Enzyme Engineering

Background:

• Peroxygenases represent a new enzyme class distinct from P450 monooxygenases.
• They catalyze cofactor-independent oxyfunctionalisation reactions, offering unique synthetic possibilities.

Purpose of the Study:

• To review recent advancements in peroxygenase research for industrial applications.
• To explore strategies for enhancing peroxygenase utility in organic synthesis.

Main Methods:

• Survey of natural peroxygenase diversity and sources.
• Analysis of protein engineering approaches for improved expression, activity, and selectivity.
• Review of enzyme immobilization techniques for enhanced stability.
• Examination of peroxygenase application in low-water reaction media.

Main Results:

• Peroxygenases exhibit diverse natural occurrence and can be engineered for specific functions.
• Immobilization strategies enhance enzyme stability and reusability.
• Application in low-water environments broadens their synthetic utility.

Conclusions:

• Peroxygenases offer significant potential as versatile biocatalysts for selective oxyfunctionalisation in organic synthesis.
• Further research is needed to fully unlock their capabilities for industrial processes.