Novel turn-on fluorescence sensor for detection and imaging of endogenous H₂S induced by sodium nitroprusside

Currently, fluorescence analysis method has a good application in the detection and imaging of biomarkers and has become an important analytical method. Although there are many fluorescent probes for detecting hydrogen sulfide(H₂S), they are mostly based on fluorophores which already existed, such as 1,8-naphthalimide, coumarin, rhodamine and their derivatives. Here, a new type of fluorescent molecule (BOTD) was synthesized and applied to the detection of H₂S. The probe BOTD could quickly and sensitively detect H₂S and turn on fluorescence. Moreover, the probe BOTD was successfully applied to the detection of exogenous and endogenous H₂S in living cells, and may be expected to become a research tool for studying H₂S-induced drugs.