Stepwise treatment of undiluted raw piggery wastewater, using three microalgal species adapted to high ammonia

A high ammonia concentration and chemical oxygen demand (COD) in piggery wastewater force it to be diluted before conventional microalgal treatment to reduce ammonia toxicity. Incomplete treatment of ammonia and COD in piggery wastewater may cause eutrophication, resulting in algal blooms. This study tried to treat raw piggery wastewater without dilution, using three strains of microalgae (Chlorella sorokiniana, Coelastrella sp. and Acutodesmus nygaardii) that outcompeted other algae under heterotrophic, mixotrophic, and autotrophic conditions, respectively, through adaptive evolution at high ammonia concentration. The three stepwise processes were designed to remove (1) small particles, COD, and phosphorus in the 1st heterotrophic C. sorokiniana cultivation, (2) ammonia and COD in the 2nd mixotrophic Coelastrella sp. cultivation, and (3) the remaining ammonia in the 3rd photoautotrophic A. nygaardii cultivation. To enhance ammonia uptake rate, each algal species were inoculated after 2-day nitrogen starvation. When the N-starved three species were inoculated at each step sequentially at 7 g/L for 2 days, the final phosphorus, COD, and ammonia removal efficiencies were 100% (16.4-0 mg/L), 92% (6820-545 mg/L), 90% (850-81 mg/L) and turbidity (99%) after total 6 days.