Related Experiment Video
Updated: Dec 11, 2025

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Solution structure of insect CSP and OBPs by NMR
Serena Leone1, Alessandro Emendato1, Roberta Spadaccini2
1Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Napoli, Italy.
Insect odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are crucial for solubilizing odorants. Solution NMR spectroscopy reveals their dynamic structures and conformational changes upon ligand binding, aiding in understanding insect olfaction.
Area of Science:
- Biochemistry
- Structural Biology
- Chemical Ecology
Background:
- Insect odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are essential for detecting olfactory cues.
- These proteins bind and transport hydrophobic odorant molecules, playing a vital role in insect chemoreception.
- OBPs and CSPs exhibit a conserved structure that undergoes significant conformational changes to accommodate various ligands.
Purpose of the Study:
- To detail experimental protocols for producing and purifying isotope-labeled OBPs and CSPs for Nuclear Magnetic Resonance (NMR) studies.
- To present comprehensive procedures for acquiring, processing, and analyzing NMR spectra.
- To illustrate the application of NMR spectroscopy in characterizing protein dynamics and ligand-binding interactions using CSP-sg4 as a model.
Main Methods:
- Production and purification of isotope-labeled recombinant OBPs and CSPs.
- Solution NMR spectroscopy for structural and dynamic analysis.
- Ligand-binding studies to investigate conformational changes.
Main Results:
- Established protocols for obtaining high-quality, isotope-labeled OBPs and CSPs suitable for NMR.
- Acquired and analyzed NMR spectra to determine structural and dynamic parameters of CSP-sg4.
- Demonstrated the ability of NMR to map ligand-induced conformational changes and binding sites.
Conclusions:
- Solution NMR spectroscopy is a powerful technique for elucidating the structure-function relationships of OBPs and CSPs.
- The described methods provide a robust framework for studying insect olfactory proteins and their interactions.
- Understanding these interactions is crucial for developing novel insect control strategies.
More Related Videos
14:55Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
08:53Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Related Concept Videos
¹H NMR: Complex Splitting
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
¹H NMR: Pople Notation
A proton...
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
NMR Spectroscopy: Chemical Shift Overview
For instance, the proton...