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Related Concept Videos

Gravimetry: Overview01:05

Gravimetry: Overview

11.0K
Gravimetric analysis is a quantitative method where the analyte is isolated and weighed directly or after conversion into a substance of known composition. Gravimetric analysis can be classified as precipitation, electrogravimetry, volatilization, and particulate gravimetry, based on the method used to isolate the analyte.
In precipitation gravimetry, the analyte is converted into a precipitate and weighed. For example, the silver content in a sample can be estimated by precipitating and...
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Electrogravimetric Analysis: Overview01:30

Electrogravimetric Analysis: Overview

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Electrogravimetric analysis measures the weight of an analyte deposited electrolytically onto a suitable working electrode. This method involves applying a potential to a pre-weighed electrode submerged in a solution, which results in the desired substance being deposited through reduction at the cathode or oxidation at the anode. The electrode's weight is recorded after deposition, and the difference in weight gives the analyte's weight in the solution.
To test the completeness of the...
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Precipitation Gravimetry01:03

Precipitation Gravimetry

11.8K
Precipitation gravimetry is based on converting an analyte into a sparingly soluble precipitate, which is separated by filtration and weighed. An ideal precipitate should be pure, insoluble, of known composition, and easily filtered from the reaction mixture.
In determining nickel by gravimetric analysis, a precipitant of ethanolic dimethylglyoxime is added to a hot nickel salt solution. This is quickly followed by the dropwise addition of dilute ammonia solution until precipitation occurs. A...
11.8K
Gravimetry: Inorganic And Organic Precipitating Agents00:49

Gravimetry: Inorganic And Organic Precipitating Agents

4.7K
In gravimetry, the precipitant is chosen carefully to obtain a pure solid that can be easily filtered. Common inorganic precipitants can be used to determine several cations and anions. In some cases, the formation of the same precipitate can be used to determine the cation and the anion. For example, the reaction of barium and chromate ions to give barium chromate is used to determine both barium and chromate. However, precipitates such as hydroxides, oxalates, and metal ammonium phosphates...
4.7K
Titrimetric Methods: Types and Commonly Used Strategies01:08

Titrimetric Methods: Types and Commonly Used Strategies

2.3K
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
2.3K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

1.3K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Related Experiment Video

Updated: Dec 11, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

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Gravimetric biosensors.

Khasim Cali1, Elena Tuccori2, Krishna C Persaud1

  • 1Department of Instrumentation and Analytical Science, The University of Manchester, Manchester, United Kingdom.

Methods in Enzymology
|August 24, 2020
PubMed
Summary
This summary is machine-generated.

Gravimetric transducers, utilizing odorant binding proteins (OBPs), can detect volatile organic compounds. Immobilization techniques on various surfaces enable the creation of sensitive gas and biosensors.

Keywords:
Gravimetric biosensorsOdorant binding proteinsQuartz crystal microbalanceSelf-assembled monolayerSurface acoustic wave sensor

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Area of Science:

  • Biotechnology
  • Biosensor technology
  • Analytical chemistry

Background:

  • Gravimetric transducers detect mass changes, enabling applications in sensing.
  • Odorant binding proteins (OBPs) are key recognition elements for volatile organic compounds (VOCs).
  • Self-assembled monolayers (SAMs) on gold and nanocrystalline diamond offer functionalized surfaces for biomolecule immobilization.

Purpose of the Study:

  • To describe methods for immobilizing OBPs onto functionalized surfaces for biosensor fabrication.
  • To explore the use of recombinant protein engineering (histidine tags) for enhanced immobilization.
  • To demonstrate the application of these methods in creating functional gravimetric biosensors.

Main Methods:

  • Immobilization of OBPs onto functionalized SAMs on gold surfaces.
  • Immobilization of OBPs onto nanocrystalline diamond surfaces.
  • Engineering recombinant OBPs with N-terminal or C-terminal histidine tags to facilitate immobilization.

Main Results:

  • Successful fabrication of functional biosensors using immobilized OBPs.
  • Demonstration of OBP immobilization on both gold-based SAMs and nanocrystalline diamond.
  • Application of histidine-tagged OBPs for improved protein attachment.

Conclusions:

  • The described immobilization methods are effective for creating gravimetric biosensors.
  • These techniques are versatile and applicable to various gravimetric transducer platforms.
  • The use of OBPs and engineered proteins offers a promising route for VOC detection.