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Updated: Dec 11, 2025

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
Published on: November 1, 2024
Intrinsic disorder-based design of stable globular proteins
Galina S Nagibina1, Tatiana N Melnik1, Ksenia A Glukhova1
1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Abstract:
Directed stabilization of globular proteins via substitution of a minimal number of amino acid residues is one of the most complicated experimental tasks. In this work, we have successfully used algorithms for the evaluation of intrinsic disorder predisposition (such as PONDR® FIT and IsUnstruct) as tools for searching for the weakened regions in structured globular proteins. We have shown that the weakened regions found by these programs as regions with highest levels of predicted intrinsic disorder predisposition are a suitable target for introduction of stabilizing mutations.
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