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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
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Cancer-Critical Genes II: Tumor Suppressor Genes01:05

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Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Dec 11, 2025

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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InVivo Cancer-Based Functional Genomics.

Chennan Li1, Andrea L Kasinski2

  • 1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA; Bindley Biosciences Center, Purdue University, West Lafayette, IN 47907, USA.

Trends in Cancer
|August 24, 2020
PubMed
Summary
This summary is machine-generated.

Mouse models and genomic tools like CRISPR and RNAi screens are crucial for understanding cancer progression and developing precision medicine. This study compares these in vivo systems to guide research and therapeutic target identification.

Keywords:
CRISPRRNAigenomic screenmouse model

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Area of Science:

  • Oncology
  • Genomics
  • Translational Research

Background:

  • Understanding tumorigenesis mechanisms is key for precision medicine.
  • In vivo mouse models offer a realistic context for studying tumor progression and therapeutic responses, surpassing cell culture limitations.
  • Functional genomic studies in mice have significantly advanced translational cancer research.

Purpose of the Study:

  • To compare various mouse models and genomic tools used for in vivo screens.
  • To highlight the strengths and limitations of different in vivo screening systems.
  • To discuss essential aspects of in vivo screen design and data analysis.

Main Methods:

  • Comparative analysis of diverse mouse models for in vivo screening.
  • Evaluation of functional genomic tools, including clustered regularly interspaced short palindromic repeats (CRISPR) and RNA interference (RNAi) screens.
  • Discussion of screen design principles and data analysis strategies.

Main Results:

  • Identification of strengths and limitations across various mouse models and genomic screening tools.
  • Insights into optimizing in vivo screen design for robust and reproducible results.
  • Guidance on effective data analysis for translational cancer research.

Conclusions:

  • In vivo mouse models and genomic screening technologies are indispensable for advancing cancer research.
  • Careful consideration of model selection, screen design, and data analysis is critical for success.
  • This comparative overview aids researchers in choosing appropriate tools for identifying therapeutic targets in precision oncology.