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Growth factor- and phorbol ester-induced changes in cell morphology analyzed by digital image processing.

Y Miyata¹, E Nishida, H Sakai

¹Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.

Experimental Cell Research

|April 1, 1988

Summary

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Computer-aided image analysis quantifies cell motility and morphology changes. Growth factors like insulin and EGF induce membrane ruffling and actin reorganization in cultured cells.

Area of Science:

Cell Biology
Biophysics
Image Analysis

Background:

Cell motility and morphological changes are crucial for biological processes.
Understanding the effects of growth factors on cellular behavior is essential.

Purpose of the Study:

To develop and apply a computer-aided image processing analysis system.
To visualize and quantify cell motility and morphological changes in living cells.
To investigate the effects of growth factors and tumor promoters on cell behavior.

Main Methods:

Computer-aided image processing and analysis (AVEC system).
Quantitative analysis using programmed trace mode.
Fluorescent phalloidin staining for actin visualization.
Video image enhancement for visualizing actin stress fibers in living cells.

Related Experiment Videos

Main Results:

Insulin, IGF-I, and EGF induced membrane ruffling in various cell lines.
Ruffling motion kinetics were quantified, showing rapid induction and decay.
Temperature and growth factor concentration influenced ruffling.
12-O-Tetradecanoylphorbol-13-acetate induced cell rounding, linked to actin reorganization.
Actin stress fiber dynamics were visualized and quantified in response to cytochalasin B.

Conclusions:

The developed computer-aided system effectively quantifies dynamic cellular changes.
Growth factors and tumor promoters induce distinct morphological alterations.
These morphological changes are associated with actin cytoskeleton reorganization.