Knee osteochondral junction imaging using a fast 3D T₁-weighted ultrashort echo time cones sequence at 3T

The osteochondral junction (OCJ) of the knee joint is comprised of multiple tissue components, including a portion of the deep layer cartilage, calcified cartilage, and subchondral bone. The OCJ is of increasing radiological interest as it may be relevant in the early pathogenesis of osteoarthritis (OA). Due to its short transverse relaxation, the OCJ is invisible to clinical MR sequences. The purpose of this study was to develop a fast 3D T₁-weighted ultrashort echo time cones sequence with fat saturation (FS-UTE-Cones) for high resolution and high contrast imaging of the OCJ on a clinical 3T scanner. First, numerical simulations were performed to investigate how the flip angle affected the signal intensities and contrasts of both short and long T₁ tissues. The results from these simulations demonstrated that higher short T₁ contrast could be achieved with higher flip angle. Next, T₁ relaxation was measured for the different layers of a human patellar cartilage sample, and the results showed that the deepest layer had a significantly shorter T₁ value than other layers. Finally, a healthy knee joint was scanned with different flip angles and the OCJ was highlighted in the T₁-weighted FS-UTE-Cones sequence using a flip angle greater than 20°. The clinical T₂-weighted and proton density-weighted FSE sequences were also included for comparison, revealing a dark OCJ region. Representative T₁-weighted FS-UTE-Cones images of the whole knee of a healthy volunteer showed high signal intensity bands in the OCJ regions of the patella, femur, and tibia. On the other hand, T₁-weighted FS-UTE-Cones imaging of the knee joints of OA patients revealed regions with reduction or loss of these high signal intensity bands in the OCJ regions, indicating abnormal OCJ tissue composition. The proposed 3D T₁-weighted FS-UTE-Cones sequence with a 3-min scan time may be very useful for demonstrating the involvement of the OCJ regions in early OA.