IL1RAP regulated by PRPRD promotes gliomas progression via inducing neuronal synapse development and neuron...

Glioma is a common fatal brain tumor that affects the central nervous system of the brain and spinal cord.

This is an original research. The morphology of M059 J cells and U373 cells were detected by microscope, cell neurite outgrowth was observed by immunofluorescence, and the expression of PRPRD and its downstream genes in HMC3 cells, M059 J cells and U373 cells were evaluated and compared with flow cytometry, immunofluorescence and Western blotting assay.

Here we show that the expression of FBP17 on the surface of glioma cells M059 J and U373 cells is more than normal cells. Overexpression of protein tyrosine phosphatase receptor-δ (PTPRD) in M059 J and U373 cells resulted in a significant increase in the S phase of the cells, while the G2 phase of the cells decreased significantly after interference with PTPRD. And PTPRD protein is mainly distributed in HMC3 cells, M059 J and U373 cytoplasm. Moreover, overexpression of PTPRD resulted in a significant increase in the expression of interleukin 1 receptor accessory protein (IL1RAP), PPFIA1 and SLITRK2, and these genes were significantly suppressed after interference with PTPRD.

This study shows that PRPRD can be used as a potential biomarker for glioma treatment. These results indicate that the PRPRD protein affects the development of neuronal synapses and neuronal differentiation by regulating IL1RAP, thereby promoting the progression of gliomas, indicating that PRPRD can be used as a potential biomarker for the treatment of gliomas.