Impact of substrate and growth conditions on microbial protein production and composition

Production of microbial protein (MP) from recovered resources - e.g. CO₂-sourced formate and acetate - could provide protein while enabling CO₂ capture. To assess the protein quality obtained from this process, pure cultures and enriched communities were selected and characterized kinetically, stoichiometrically and nutritionally. Growth on acetate resulted in up to 5.3 times higher maximum specific growth rate (μ_max) than formate (i.e. 0.15-0.41 h^-1 for acetate compared to 0.061-0.29 h^-1 for formate at pH = 7). The protein content was a function of the growth phase, with the highest values during stationary phase, ranging between 18 and 82%_CDW protein depending on the organism and substrate. The negative correlation between biomass productivity and protein content indicated a trade-off between production rate and product quality. The final product (i.e. dried MP) quality was in most cases superior to soybean and all cultures were rich in threonine, phenylalanine and tyrosine, regardless of the carbon source.