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Dual functionalized chitosan based composite hydrogel for haemostatic efficacy and adhesive property
Gopendra Singh1, Aradhana Nayal2, Sahil Malhotra1
1Centre for Biomedical Engineering, Indian Institute of Technology-Delhi, Hauz Khas, New Delhi, 110016, India; Biomedical Engineering Unit, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
Carbohydrate Polymers
|August 25, 2020
Summary
This study developed a dual-functionalized chitosan hydrogel dressing for effective hemorrhage control. The advanced hemostatic dressing shows superior performance in clotting, adhesion, and fluid absorption while reducing inflammation.
Area of Science:
- Biomaterials Science
- Hemostasis Research
- Wound Management Technologies
Background:
- Hemorrhage control remains a critical challenge in medical emergencies.
- Existing hemostatic dressings often have limitations in efficacy, adhesion, or biocompatibility.
- Chitosan-based materials offer promising properties for wound healing and hemostasis.
Purpose of the Study:
- To develop a dual-functionalized chitosan hydrogel dressing with enhanced hemostatic properties.
- To investigate the hemostatic, mechanical, absorption, and anti-inflammatory potential of the novel hydrogel.
- To evaluate the biocompatibility and non-toxicity of the optimized hydrogel dressing.
Main Methods:
- Formulation of a hydrogel using quaternized chitosan, phosphorylated chitosan, tannic acid, and poly-Ɛ-lysine.
- Assessment of hemostatic activity through clotting time, prothrombin time, and activated partial thromboplastin time.
- Evaluation of platelet activation, adhesion strength, fluid absorption, and mechanical properties.
- Determination of proinflammatory potential by measuring Tumor Necrosis Factor-α and Interleukin-6 levels.
- Cytotoxicity testing using L929 fibroblasts and in-vivo studies in Wistar rats.
Main Results:
- The optimized hydrogel demonstrated superior hemostatic activity with a clotting time of 225 ± 5 s.
- Significantly enhanced platelet activation, adhesion strength (3x higher than Axiostat), and fluid absorption (14x in 12h).
- Improved mechanical properties, faster coagulation, and reduced proinflammatory markers (TNF-α and IL-6) compared to a commercial dressing.
- Cytotoxicity and in-vivo studies confirmed the hydrogel's non-toxic, cytocompatible, and biocompatible nature.
Conclusions:
- The dual-functionalized chitosan hydrogel dressing presents a highly effective solution for hemorrhage control.
- The formulation exhibits excellent hemostatic, mechanical, and absorption capabilities with reduced inflammation.
- The developed hydrogel is safe, non-toxic, and biocompatible, indicating its potential for clinical application in wound management.
