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Circ-SLC8A1 regulates osteoporosis through blocking the inhibitory effect of miR-516b-5p on AKAP2 expression

Cheng Lin1, Wenjun Zhong1, Wei Yan1

  • 1Department of Orthopedics, Clinic Medical College of Anhui Medical University, the First People's Hospital of Chuzhou City, Chuzhou, Anhui, China.

The Journal of Gene Medicine
|August 25, 2020
PubMed
Abstract

Insights

Circular RNA SLC8A1 (circ-SLC8A1) is reduced in osteoporosis and counteracts miR-516b-5p's effects on AKAP2. This finding offers a potential new treatment strategy for osteoporosis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Osteoporosis is a bone disease marked by bone loss and altered bone metabolism.
  • Circular RNAs (circRNAs) are emerging regulators in human diseases, but their role in osteoporosis is not well understood.

Purpose of the Study:

  • To investigate the expression and mechanism of circRNAs in osteoporosis.
  • To explore the role of circ-SLC8A1 in the context of osteoporosis.

Main Methods:

  • Bioinformatic prediction of microRNA binding sites.
  • Dual luciferase assays to confirm target interactions.
  • Quantitative reverse transcriptase-polymerase chain reaction to assess gene expression.
  • Cell staining assays to evaluate osteogenic differentiation.

Main Results:

  • Circ-SLC8A1 expression was significantly decreased in an osteoporosis mouse model.
  • Circ-SLC8A1 negatively regulated miR-516b-5p expression.
  • Overexpression of circ-SLC8A1 inhibited miR-516b-5p's suppressive effect on AKAP2.

Conclusions:

  • Circ-SLC8A1 inhibits miR-516b-5p's action on AKAP2, thereby influencing osteoporosis.
  • Circ-SLC8A1 presents a potential therapeutic target for osteoporosis treatment.
Keywords:
AKAP2circ-SLC8A1miR-516b-5posteoporosis

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