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Cell-substrate adhesion during Trypanosoma cruzi differentiation.

M C Bonaldo¹, T Souto-Padron, W de Souza

¹Fundacao Oswaldo Cruz (FIOCRUZ), Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brazil.

The Journal of Cell Biology

|April 1, 1988

Summary

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The Journal of parasitology·2012

Trypanosoma cruzi epimastigotes adhere to surfaces before transforming into infective metacyclic trypomastigotes. Specific surface proteins are expressed during this crucial adhesion step in metacyclogenesis.

Area of Science:

Parasitology
Cell Biology
Molecular Biology

Background:

Trypanosoma cruzi causes Chagas disease.
Metacyclogenesis is the transformation of epimastigotes to infective trypomastigotes.
In vitro models allow study of this transformation.

Purpose of the Study:

To investigate the in vitro transformation of Trypanosoma cruzi epimastigotes to metacyclic trypomastigotes.
To identify parasite surface molecules involved in the transformation process.

Main Methods:

In vitro culture of Trypanosoma cruzi under defined conditions.
Scanning and transmission electron microscopy.
PAGE analysis of radiolabeled proteins.
Antiserum characterization.

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Main Results:

Epimastigotes adhere to a surface before transforming into metacyclic trypomastigotes.
Metacyclic trypomastigotes are released into the supernatant.
Adhered parasites express specific 45-50 kD surface proteins (pI 4.8).
These proteins are recognized by antiserum against adhered parasites.

Conclusions:

Epimastigote adhesion to a substrate is a prerequisite for metacyclogenesis.
Specific surface proteins are involved in the adhesion phase of transformation.
This in vitro process may mimic natural metacyclogenesis in the insect vector.