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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Cell Specific Gene Expression01:58

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Chromatin Position Affects Gene Expression02:35

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
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Signal Sequences and Sorting Receptors01:41

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Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
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Related Experiment Video

Updated: Dec 11, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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Evaluating the Effects of Cell Sorting on Gene Expression.

Andrew Box1, Monica DeLay2, Scott Tighe3

  • 1Stowers Institute for Medical Research, Kansas City, Missouri, USA.

Journal of Biomolecular Techniques : JBT
|August 25, 2020
PubMed
Summary

Cell sorting minimally impacts cell gene expression and cell cycle. These effects are reduced after culturing, ensuring sorted cells are suitable for downstream applications like gene expression assays.

Keywords:
RNA microarrayflow cytometryimmune cells

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Molecular Biology

Background:

  • Cell sorting is crucial for isolating pure cell populations for downstream analysis.
  • Ensuring minimal cellular impact from sorting is vital for assay validity.
  • Previous research often focused on specific cell types or conditions.

Purpose of the Study:

  • Establish best practice guidelines for cell sorting.
  • Minimize cell stress, perturbation, and injury during sorting.
  • Evaluate the impact of sorting parameters on cell gene expression and cell cycle.

Main Methods:

  • Assessed effects of nozzle size, sample pressure, UV exposure, and instrument type.
  • Utilized established cell lines and primary cells.
  • Conducted experiments across multiple flow cytometry shared facilities.

Main Results:

  • Nozzle size, pressure, UV exposure, and instrument type showed minor effects on gene expression.
  • Subsequent culturing diminished any observed gene expression changes.
  • Cell sorting demonstrated minimal impact on downstream cellular applications.

Conclusions:

  • Cell sorting parameters have limited effects on cell gene expression and cell cycle.
  • Culturing effectively mitigates minor sorting-induced cellular alterations.
  • The cell sorting process itself has minimal impact on the suitability of cells for downstream applications.