Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.1K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.2K
2.2K
The DNA Replication Fork01:02

The DNA Replication Fork

39.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
39.7K
The DNA Replication Fork01:02

The DNA Replication Fork

17.6K
17.6K
Homologous Recombination02:31

Homologous Recombination

61.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
61.7K
The Replisome03:01

The Replisome

37.6K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Typhoid Toxin of Salmonella enterica Induces ISG15 Responses Mediating Host Cell Survival and Counteracting Intracellular Infection.

Molecular microbiology·2026
Same author

SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia.

Cell death & disease·2024
Same author

MRNIP limits ssDNA gaps during replication stress.

Nucleic acids research·2024
Same author

Development and Optimisation of Tumour Treating Fields (TTFields) Delivery within 3D Primary Glioma Stem Cell-like Models of Spatial Heterogeneity.

Cancers·2024
Same author

Ex-vivo drug screening of surgically resected glioma stem cells to replace murine avatars and provide personalise cancer therapy for glioblastoma patients.

F1000Research·2023
Same author

DNA damage response inhibitors enhance tumour treating fields (TTFields) potency in glioma stem-like cells.

British journal of cancer·2023
Same journal

Taphonomic analysis at Liang Bua reveals the behavioral and technological capabilities of <i>Homo floresiensis</i>.

Science advances·2026
Same journal

Targeting granule initiation and amyloplast structure to create giant starch granules in wheat.

Science advances·2026
Same journal

A meta-analysis of carbon losses and gains from tropical moist forest degradation and regeneration.

Science advances·2026
Same journal

Ancient DNA reveals elite dynastic rule among Iron Age Eurasian Steppe nomads.

Science advances·2026
Same journal

Targeting astrocytic Dp71 attenuates BBB disruption after traumatic brain injury through WTAP-associated m<sup>6</sup>A regulation of MMP2.

Science advances·2026
Same journal

Pancreatic α cells are required for nutrient homeostasis by regulating dynamic β cell networks in islets.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Dec 11, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.8K

MRNIP is a replication fork protection factor.

L G Bennett1, A M Wilkie1, E Antonopoulou1

  • 1North West Cancer Research Institute, School of Medical Sciences, Bangor University, Bangor LL57 2UW, UK.

Science Advances
|August 25, 2020
PubMed
Summary
This summary is machine-generated.

MRNIP is a novel protein that protects DNA replication forks by inhibiting MRE11 exonuclease activity. Its absence leads to DNA degradation, genomic instability, and increased sensitivity to chemotherapy.

More Related Videos

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

9.4K
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
10:32

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

7.3K

Related Experiment Videos

Last Updated: Dec 11, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.8K
Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

9.4K
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
10:32

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

7.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • DNA Replication

Background:

  • Replication fork remodeling is crucial for the DNA replication stress response.
  • RAD51 and BRCA1/2 protect nascent DNA at reversed forks, but MRE11-mediated degradation can occur if this fails, causing genomic instability.

Purpose of the Study:

  • To investigate the mechanisms regulating MRE11 function at reversed replication forks.
  • To identify novel factors involved in fork protection and genome integrity.

Main Methods:

  • Identified MRNIP as an MRE11-binding protein.
  • Assessed the effect of MRNIP loss on replication fork progression and DNA degradation.
  • Evaluated MRE11 exonuclease activity regulation by MRNIP.

Main Results:

  • MRNIP directly binds MRE11 and represses its exonuclease activity.
  • Loss of MRNIP leads to impaired replication fork progression and MRE11-dependent degradation of reversed forks.
  • MRNIP deficiency results in underreplicated DNA, chemosensitivity, and chromosome instability.

Conclusions:

  • MRNIP is a novel regulator of MRE11 at reversed replication forks.
  • Regulation of MRE11 nuclease activity by MRNIP is essential for protecting nascent DNA and maintaining genome integrity.