An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher
Masahiro Oe1, Koji Miki1, Kouichi Ohe1
1Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. kojimiki@scl.kyoto-u.ac.jp ohe@scl.kyoto-u.ac.jp.
Abstract:
We developed a new class of turn-on fluorescent probes for an esterase. After the esterase-mediated hydrolysis produced carboxylate (as a fluorescence activator), the fluorescence intensity was markedly increased through the detachment of a quencher moiety from the quenched Cy5 fluorophore. Because the probes based on this new activator-induced quencher-detachment (AiQd) adopt a non-immolative linker between the cleavable site and the fluorophore, the rate of the enzymatic reaction is greatly improved, without the generation of any by-products.
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