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Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
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Isolation and Propagation of Coronaviruses in Embryonated Eggs
1Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. jsguy@ncsu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2020
Summary
Embryonated eggs support diverse virus replication, particularly avian coronaviruses like infectious bronchitis virus (IBV) and turkey coronavirus (TCoV). Different inoculation routes are optimal for each virus, highlighting the egg
Area of Science:
- Virology
- Embryology
- Veterinary Science
Background:
- Embryonated eggs offer a versatile system for virus propagation due to diverse cell types.
- Avian coronaviruses, including infectious bronchitis virus (IBV) and turkey coronavirus (TCoV), are studied using embryonated eggs.
Purpose of the Study:
- To explore the utility of embryonated eggs as a host system for coronaviruses.
- To detail optimal inoculation routes for IBV and TCoV propagation in embryonated eggs.
Main Methods:
- Inoculation of embryonated chicken eggs with IBV and TCoV.
- Utilizing different routes (allantoic, amniotic) based on viral tropism.
- Quantification of virus titers in membranes and fluids.
Main Results:
- IBV efficiently replicates in the chorioallantoic membrane via the allantoic route, yielding high titers.
- TCoV replication is restricted to embryonic intestinal and bursal epithelium, requiring amniotic inoculation.
- Embryonated eggs demonstrate potential for novel coronavirus detection and characterization.
Conclusions:
- Embryonated eggs are a valuable host system for avian coronavirus research.
- Specific inoculation routes are critical for efficient isolation and propagation of IBV and TCoV.
- This system holds promise for the study of emerging coronaviruses.
