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In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

Tran Thi Nhu Thao1,2,3, Fabien Labroussaa2,4, Nadine Ebert1,2

  • 1Institute of Virology and Immunology IVI, Bern, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2020
PubMed

Keywords:
CoronavirusFull-length cDNA cloneHomologous recombinationRNA virusReverse geneticsSaccharomyces cerevisiaeSynthetic genomicsTransformation-associated recombination (TAR) cloning

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