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Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

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Updated: Dec 11, 2025

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Using Yeast to Identify Coronavirus-Host Protein Interactions.

Stuart Weston¹, Matthew Frieman²

¹Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA. sweston@som.umaryland.edu.

Methods in Molecular Biology (Clifton, N.J.)

|August 25, 2020

Summary

This study introduces a yeast screening system to find host genes impacting mammalian virus replication. This adaptable method aids in understanding virus-host interactions for any emerging virus.

Keywords:

Host factors Host–virus interaction Suppressor screening Yeast

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Area of Science:

Virology
Molecular Biology
Genetics

Background:

Understanding virus-host interactions is crucial for antiviral development.
Mammalian virus replication mechanisms are complex and require host cell machinery.
Current screening methods may be limited by virus containment and culturing requirements.

Purpose of the Study:

To develop a versatile yeast-based screening system for identifying eukaryotic genes involved in mammalian virus replication.
To leverage Saccharomyces cerevisiae for efficient genome-wide screening of host-virus interactions.
To provide a platform applicable to any virus, irrespective of biosafety level or culturability.

Main Methods:

Utilizing Saccharomyces cerevisiae as a model organism for its ease of use and biosafety level 2 compatibility.
Implementing inducible expression of individual viral proteins to identify detrimental phenotypes in yeast.
Employing yeast knockout and overexpression libraries for genome-wide screening of host suppressor genes.
Analyzing yeast 'hits' to generate a narrowed list of candidate genes for further investigation.

Main Results:

Demonstrated the feasibility of using yeast to screen for host factors influencing viral replication.
Established a system adaptable for identifying genes involved in the replication of diverse mammalian viruses.
Identified potential host genes that modulate viral replication through suppressor phenotypes in yeast.

Conclusions:

Saccharomyces cerevisiae provides a powerful and adaptable platform for dissecting virus-host interactions.
The developed screening system facilitates the identification of host genes essential for viral replication.
This approach advances the understanding of viral replication mechanisms and can accelerate antiviral research for emerging infectious diseases.