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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
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Visualizing Coronavirus Entry into Cells
Aleksandra Milewska1, Katarzyna Owczarek1, Artur Szczepanski1
1Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Abstract:
Coronavirus entry encompasses the initial steps of infection, from virion attachment to genome release. Advances in fluorescent labeling of viral and cellular components and confocal imaging enable broad spectrum studies on this process. Here, we describe methods for visualization of coronavirus entry into immortalized cell lines and 3D tissue culture models.
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