[Orthotopic liver transplantation in miniature pigs: some technical considerations in recipient]

We compared temporary aortic occlusion technique (TAO) with venous bypass in anhepatic phase of the recipient in the liver transplants in miniature pig, using cuff technique in connecting the portal vein and/or the inferior vena cava. Twenty four hour survival rate after liver transplant by TAO in anhepatic phase was almost the same as that by venous bypass. There was no difference in the posttransplant changes of serum transaminase and coagulating factors between the two groups above. Profound hypotension and metabolic acidosis were encountered on the release of occluded aorta. Rapid blood transfusion and fluid infusion were necessary with the administration of bicarbonate to keep the blood pressure constant. Though we had to take special care to stabilize the general condition during the operation, the operation technique with TAO was quite simple. The hemodynamic state of the venous bypass group was also stable, but the operation was more complex and troublesome. Cuff technique was useful in connecting veins and recipients' survival rate with cuff technique was better than that with suturing method but the anastomosis by cuff technique always resulted in chronic stenosis and our new method, temporary cuff technique, was effective to prevent chronic stenosis.